UM101-200µg Anti-Ub Tube1, GST

UM401: TUBE 1 (Agarose)

$555.00

In stock

SKU: UM-0401-1000 Category: Tags: , ,

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Agarose coupled TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.

Info

Tag

His6-SUMO

Molecular Weight

 N/A

Physical State

Liquid

Quantity

1 mL

Concentration

Variable

Storage

-20°C, avoid freeze/thaw cycles, avoid storing below this temperature

Additional information

SKU

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Applications

  • Pull down of polyubiquitylated proteins from cell lines, tissues and organs
  • Isolation of polyubiquitinated proteins for proteomic studies
  • Protection of polyubiquitylated proteins from deubiquitylation and degradation by the proteasome

Benefits

  • One step recovery of polyubiquitinated proteins
  • Efficient isolation of polyubiquitinated proteins from TUBEs
  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
  • Protect polyubiquitin chains without inhibitors specific to either the proteasome or deubiquitylase activity

Documents

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