LifeSensors has developed the SUMOstar™ tag, a modified version of the SUMOpro tag that extends the benefits of SUMO to mammalian hosts. Like SUMOpro, it increases the expression and solubility of your protein of interest while allowing proper post-translational modifications (PTMs) within the cells. However, the SUMOstar™ tag has been uniquely engineered to resist endogenous SUMO proteases found in eukaryotic systems that might otherwise cleave the tag prematurely. The SUMOstar™ tag remains tethered to the protein throughout the purification process. Precise C-terminal removal of the tag simply requires end user-induced cleavage by engineered SUMOstar™ proteases. The native N-terminal residues of the protein are completely preserved following cleavage, which may be important for its biological activity (e.g. cytokines).
We offer both yeast and human versions of SUMOstar™ depending on your target species of interest.