Fusion with SUMO (Small Ubiquitin Like Modifier) has revolutionized the way difficult to express proteins are made. The SUMO tag offers advantages over traditional expression methods. Mechanisms and benefits of SUMO mediated enhanced expressions include:
- chaperoning properties of SUMO dramatically enhance translation of proteins/polypeptide
- presence of globular protease resistant SUMO domain stabilizes proteins,
- the most efficient SUMO protease reduce the cost of native protein production.
- SUMO protease cleaves any amino acid sequence at the junction generating desired N-termini, especially important for cytokine, growth factors and chemokine to restore biological activity.