LifeSensors’ UbiQuant ELISA assay can measure the concentration of total ubiquitin in cell and tissue lysates or measure the concentration of ubiquitinated proteins of interest. Ubiquitylated proteins are captured by an immobilized ubiquitin affinity matrix. Subsequently, unbound proteins are washed away, and total free ubiquitin and ubiquitin conjugates are quantified using an anti-ubiquitin antibody, in conjunction with traditional ELISA detection reagents. There are many advantages to this R&D service. UbiQuant ELISA allows for testing the effect of multiple compounds or conditions on the ubiquitylation levels in cell/tissue lysates. Additionally, this assay is useful for monitoring the effects of broad-spectrum compounds such as proteasome inhibitors on total ubiquitin levels.
- Determine changes in the level of substrate poly- ubiquitination
- Chain specific ubiquitin detection
- Uses an antibody that specifically binds to the protein of interest (or in some cases, ubiquitin)