UM601: TUBE 1 (FLAG)

LifeSensors UM601 TUBE 1 (FLAG) offers up to 1000-fold higher affinity for isolating polyubiquitylated proteins, enhancing ubiquitin research without overexpressing tagged ubiquitins.

$575.00 – $1,799.00

SKU UM-0601 Categories All, Pan-Selective TUBEs Tags Pan-selective, Pull down, Tandem Ubiquitin Binding entities

Product Advantages

Applications

Pull down of poly-ubiquitylated proteins from cell and tissue lysates
Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome

Benefits

Up to 1000-fold higher affinity than the single UBA domain
Avoid overexpression of affinity-tagged ubiquitins for pull downs
Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins

Based on UBA domains known to possess a high affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs have been demonstrated to protect polyubiquitinated proteins from both deubiquitination and proteasome-mediated degradation, allowing for the detection of relatively low abundant proteins that cannot be detected with current technologies. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.

Info

Tag	FLAG
Molecular Weight	28.2 kDa
Physical State	Liquid
Quantity	200 µg and 1 mg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles

Datasheet
Manual

Datasheet
Manual

Applications

Pull down of poly-ubiquitylated proteins from cell and tissue lysates
Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome

Benefits

Up to 1000-fold higher affinity than the single UBA domain
Avoid overexpression of affinity-tagged ubiquitins for pull downs
Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins