The SUMOpro tag system is based off our SUMO protein expression platform to maximize the yield of soluble and functional proteins with a native N-terminus. Precise tag removal relies on LifeSensors’ highly specific SUMO proteases, which recognize the tertiary structure of the tag at the N-terminus of the partner protein and cleaves the junction at discrete residues outside the protein sequence. Therefore, SUMO proteases are superior to other proteases commonly used in prokaryotic expression systems that may non-specifically cleave within the protein of interest or leave extra residues behind. By linking a His6 tag to both the SUMOpro fusion tag and SUMO protease, the system allows efficient and rapid purification of untagged proteins using immobilized metal affinity chromatography. SUMOpro fusion, thus, makes purifying your protein easy and affordable.
LifeSensors offers both yeast (SUMOpro) and human (SUMOpro3) tags with corresponding SUMO proteases 1 and 2, respectively.