UM101-200µg Anti-Ub Tube1, GST

UM301: TUBE 1 (Biotin)

$435.00

In stock

SKU: UM-0301-0200 Category: Tags: , ,

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state.  Biotin-TUBEs allow for the detection of polyubiquitin and polyubiquitylated proteins by ligand blotting (“far Western”) without heating the membrane. This reagent is a superior alternative to traditional polyubiquitin immunodetection techniques, such as anti-ubiquitin IgGs.

This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Tag

Biotin

Molecular Weight

Physical State

Liquid

Quantity

200 µg

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

 

*Biotinylation is restricted to the His6-SUMO tag to avoid compromising the affinity of the TUBE moiety for polyubiquitin.

Additional information

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Applications

  • Detection of polyubiquitylated proteins by ligand blotting
  • Pull down of polyubiquitylated proteins from cell lines, tissues and organs using a variety of readily available avidin supports
  • In situ labeling for detection of polyubiquitin by histochemistry

Benefits

  • Replace ubiquitin antibodies for western blotting; avoid membrane boiling
  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs

Documents