PROTACs (PROteolysis Targeting Chimeras) are heterobifunctional molecules that combine a target-binding warhead with an E3 ligase-binding moiety, resulting in the formation of a ternary complex between the target protein and the E3 ligase to induce ubiquitination and degradation. It is increasingly clear that the length and composition of the linker connecting the two ligands plays a crucial role in the physicochemical properties and biological activity of PROTACs. The PROTAC field is rapidly evolving and is currently undergoing an important transition from synthetically manageable alkyl and polyethylene glycol linkers to more complex functional linkers. Traditionally, ternary complex formation has been assessed using cell-free proximity ligand assays such as TR-FRET, ALPHA screening assays, and SPR-based methods. 

Studies in recent years have shown that protein degradability is strongly influenced by intrinsic characteristics of the protein, especially the protein’s endogenous “Ubiquitination Potential”. Hence correlating Ternary complex formation with ubiquitination potential is crucial in accurately characterizing PROTAC efficiency and helping chemists rationally design. LifeSensors offers wide array of services to validate PROTAC ternary complex, PROTAC ubiquitination and determining binding affinities (SPR).

Service Highlights

  • Multiple approaches to rank PROTAC variants
  • Fast and Sensitive
  • Correlate activity with orthogonal assays
  • Evaluate relative population of ternary complexes and correlate with ubiquitination potential
  • Access to ~ 30 E3 ligases to study selectivity

Related Products

PROTAC® Assay Plate

Pre-coated and blocked 96-well or 384-Well strip PROTAC assay plate.

UbiQuant™ Kit

UbiQuant™ is a sandwich assay that allows for the absolute measurement of ubiquitin in a cell lysate.


Technology to specifically isolate polyubiquitylated proteins from cell lysates and tissues using Tandem Ubiquitin Binding Entities (TUBEs).