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UbiTest Substrate Validation

LifeSensors’ UbiTest is a medium-throughput platform for measuring the polyubiquitylation levels of protein(s) of interest (POI). UbiTest utilizes TUBE technology to enrich polyubiquitylated proteins. This fraction is analyzed by immunoblotting techniques with and without pan-selective DUB digestion. The appearance or increase in the intensity of the band corresponding to the POI indicates that the POI is polyubiquitylated in the studied condition. Identification of the type of ubiquitin linkage can provide insights into the cellular functions of E3s and substrates.

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UM101-200µg Anti-Ub Tube1, GST

UM401: TUBE 1 (Agarose)

LifeSensors UM401 is an agarose-coupled Tandem Ubiquitin Binding Entity (TUBE 1) that efficiently captures polyubiquitinated proteins for proteomic and Western blot analysis.

$1,002.00