The SUMOpro tag system was developed from SUMO to maximize the yield of soluble and functional proteins with a native N-terminus. We can extend the benefits of our SUMOpro tag to mammalian hosts, increasing the expression and solubility of your protein while ensuring proper post-translational modifications (PTMs) within the cell. Precise tag removal relies on LifeSensors’ highly specific SUMO proteases, which recognizes the tertiary structure of the tag at the N-terminus of the partner protein and cleaves the junction at discrete residues outside the protein sequence. LifeSensors offers both yeast (SUMOpro) and human (SUMOpro-3) tags with corresponding SUMO proteases 1 and 2, respectively, for mammalian expression. By linking a His6 tag to both the SUMOpro fusion tag and SUMO protease, the system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography. SUMOpro fusion, thus, makes purifying your protein easy and affordable.
Service Highlights
- Convenient, directional cloning of your gene-of-interest in frame with the SUMOpro tag
- Dramatically enhances protein expression and solubility, reducing costs
- Precise tag removal following protease cleavage
Related Products
SUMO Protease 1
SUMO-GFP is a recombinant protein that contains yeast SUMO fused to a control polypeptide (GFP). This fusion protein is used as a positive control for SUMO Protease 1 activity.
SUMO Protease 2
hSUMO3-GFP is a recombinant fusion protein that contains the human SUMO3 tag followed by a control polypeptide (GFP). This fusion protein is used as a positive control for SUMO Protease 2 activity.
