The Ubiquitin Proteasome System (UPS) controls the principal functions of almost all the cellular proteins of human cells and failures in this system often contribute directly or indirectly to the pathogenesis of many diseases, including cancer, inflammation, and neurodegeneration. There are ~100 Deubiquitinases (DUBs) encoded in human genome and the functions of most of these DUBs remain unknown. Identification of substrates of individual DUBs is an essential step to unravel cellular functions of DUBs. Lifesensors can help you identify the substrates of your preferred DUBs.
- In vitro and in cell assays to identify DUB substrates
- Human protein array enables identification of direct deubiquitination targets
- Substrate Trapping and Proteomic analysis to identify endogenous substrates
- Validation of substrates in orthogonal assays
Coated polymeric high-capacity magnetic beads to allow superior enrichment of polyubiquitinated proteins along with minimizing non-specific binding to proteins in tissue and cellular lysates.
TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance.
Monoclonal antibodies that recognize polyubiquitylated proteins and free ubiquitin. VU-1 has been shown to recognize all ubiquitin linkages (mono, K6, K11, K27, K29, K33, K48, K63, and linear) by Western blotting. VU-1 is an excellent antibody for immunostaining applications generating robust ubiquitin detection and low background with an ability to detect subcellular ubiquitin localization.