Brefeldin A is a cell permeable fermentation product from Penicillium brefeldianum. It is a lactone antibiotic and ATPase inhibitor for protein transport with an IC50 of 0.2 μM in HCT 116 cells. Brefeldin A has been widely used as specific inhibitor of protein translocation from endoplasmic reticulum to Golgi. It blocks secretion and prevents the assembly of non-clathrin-coated buds on Golgi cisternae. Brefeldin A also inhibits guanine nucleotide-exchange proteins BIG1 and BIG2, activators of ADP-ribosylation factors. It induces apoptosis independently of p53 and activates sphingomyelin metabolism.
|Purity||>99.0% by HPLC|
|Quantity||5 mg, 25 mg, 50 mg|
|Molecular Weight||280.4 g/mol|
|Physical State||Lyophilized powder|
|Solubility||100 mg/mL in DMSO; or 25 mg/ml in warm ethanol|
|Storage||-20°C, avoid freeze/thaw cycles|