UM101-200µg Anti-Ub Tube1, GST

UM502T: TUBE 2 (TAMRA)

$535.00

In stock

SKU: UM-0502T-0050 Category: Tags: , ,

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. TAMRA-TUBE 2 has a single TAMRA fluorophore (Exc. = 540 nm, Emm. = 578 nm) attached to the fusion tag of TUBE 2 (Cat. No. UM202). Since the fluorophore is attached to the tag, it does not affect binding of the tandem ubiquitin binding domains to polyubiquitin chains.

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Info

Tag

His6

Molecular Weight

39 kDa

Physical State

Liquid

Quantity

50 µg

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

Additional information

SKU

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Applications

  • Cytochemical staining of polyubiquitylated proteins for fluorescence microscopy

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