UM502F: TUBE 2 (Fluorescein)

LifeSensors UM502F is a fluorescein-labeled Tandem Ubiquitin Binding Entity (TUBE) for selective enrichment and detection of polyubiquitinated proteins in proteomics and drug discovery research.

$744.00

SKU UM-0502F-0050 Categories Pan-Selective TUBEs, TUBEs: Ubiquitin Affinity Matrix Tags Pan-selective, Pull down, Tandem Ubiquitin Binding entities

In stock

Product Advantages

Applications

Cytochemical staining of polyubiquitylated proteins for fluorescence microscopy

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” protein in its polyubiquitin state. FLR-TUBE2 has an average of three fluorescein moieties (Exc. = 490 nm, Emm. = 520 nm) attached to the fusion tag of TUBE 2 (Cat. No. UM202). Since the fluorophore is attached to the tag, it does not affect binding of the tandem ubiquitin binding domains to polyubiquitin chains. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.