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UM401M: TUBE 1 (Magnetic Beads)
LifeSensors UM401M TUBE 1 Magnetic Beads enable efficient isolation and enrichment of polyubiquitinated proteins from cell and tissue extracts in a single-step, centrifuge-free process.
Pull down of polyubiquitylated proteins from cell lines, tissues and organs
Isolation of polyubiquitinated proteins for proteomic studies
Protection of polyubiquitylated proteins from deubiquitylation and degradation by the proteasome
Benefits
One step recovery of polyubiquitinated proteins
No centrifuge needed and lower background
Efficient isolation of polyubiquitinated proteins from TUBEs
Up to 1000-fold higher affinity than the single UBA domain
Avoid overexpression of affinity-tagged ubiquitins for pull downs
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Magnetic beads coupled to TUBE 1 allow for efficient recovery of polyubiquitinated proteins in a single step without centrifuge. The magnetic TUBEs allows more complete removal of the unbound supernatant, which leads to lower background. Polyubiquitinated proteins can easily be separated from TUBEs for proteomic studies or identification by Western blotting.
