UM504F: K63 TUBE (Fluorescein)

Specifications

Fluorescein-labeled (FLR) K63 TUBE binds K63-linked polyubiquitin with a 1000 to 10,000-fold preference for K63 over other linkage types and provides a sensitive and cost-effective tool for determining the abundance of K63-linked polyubiquitin in the cell, or identifying the linkage-type associated with a particular protein of interest. FLR-K63 TUBE consists of multiple ubiquitin interacting motifs separated by rigid linkers, presumably increasing its affinity to extended K63 polyUb chains. FLR- K63 TUBE is useful for detection and characterization of K63-linked polyubiquitylated proteins by ‘far Western’ blotting and in situ detection (histo- or cyto-chemical staining). FLR-K63 TUBE has one fluorescein molecule conjugated to a single, defined location within the protein.

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