UM201-1MG Anti-Ub TUBE 1, His6

UM400M: Control Magnetic Beads (negative control)

$152.25

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SKU: UM-0400M-1000 Categories: , , Tag:

Specifications

For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitinated proteins in conjunction with magnetic beads-conjugated TUBEs (UM401M and UM402M). Control magnetic beads are generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control magnetic beads (1 mL) are supplied as a slurry in PBS, pH 7.2.

Info

Tag N/A
Molecular Weight
Physical State Liquid
Quantity 1 mL
Concentration Variable
Storage +4°C, avoid storing below this temperature

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Applications

  • Negative control for pull-down experiments
  • “Pre-clearing” of cell lysates prior to pull-down experiments
  • General recommendations: Pre-clear or use as a negative control for 1ml of cell lysate (3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr (4°C). Individual conditions may have to be optimized by the end user.

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