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UM400M: Control Magnetic Beads (negative control)
LifeSensors UM400M is a negative control magnetic bead used in ubiquitin affinity assays, ensuring specificity in ubiquitin-binding protein studies and proteomic analyses.
“Pre-clearing” of cell lysates prior to pull-down experiments
General recommendations: Pre-clear or use as a negative control for 1ml of cell lysate (3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr (4°C). Individual conditions may have to be optimized by the end user.
For use in “pre-clearing” cell lysates, as well as a negative control for the pulldown of polyubiquitinated proteins in conjunction with magnetic beads-conjugated TUBEs (UM401M and UM402M). Control magnetic beads are generated by blocking an activated matrix (identical to that used for TUBEs conjugation) with ethanolamine. Control magnetic beads (1 mL) are supplied as a slurry in PBS, pH 7.2.
“Pre-clearing” of cell lysates prior to pull-down experiments
General recommendations: Pre-clear or use as a negative control for 1ml of cell lysate (3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr (4°C). Individual conditions may have to be optimized by the end user.
