UM202: TUBE 2 (His6)




Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitinated state. The ubiquitination state of certain cellular proteins can be directly related to various diseases including neurodegeneration, inflammation, and cancer. However, ubiquitinated proteins are intrinsically unstable, which precludes the characterization of the ubiquitination state in many cases. TUBEs will fill this void in ubiquitin research, allowing the identification of ubiquitin-modified proteins that cannot be detected with current technologies. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cells, tissues and organs. The affinity of TUBE 2 for K63 linked tetra-ubiquitin and K48 tetra-ubiquitin linked chains is equivalent.

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Tag His6
Molecular Weight 38.5 kDa
Physical State Liquid
Quantity 200 µg, 1 mg
Concentration Variable
Storage -80°C, avoid freeze/thaw cycles

Additional information


200 µg, 1 mg


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  • Pull down of poly-ubiquitylated proteins from cell and tissue lysates
  • Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome


  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
  • Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
  • Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins