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UB218: UBE2N

LifeSensors UB218 (UBE2N) is an E2 ubiquitin-conjugating enzyme that assembles K63-linked polyubiquitin chains, key in non-degradative ubiquitin signaling pathways like DNA repair and immune response. Ideal for research in ubiquitination and cellular signaling

Price range: $245.00 through $439.00

Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme
UBE2N (homologous to yeast Ubc13) is an E2 conjugating enzyme that forms a heterodimeric complex with the ubiquitin E2 variant (UEV) UBE2V2 (also known as MMS2 or UEV-2). This complex is responsible for the ubiquitylation of target substrates along with E1 and E3 enzymes. The UBE2N/UBE2V2 complex assembles K63-linked polyubiquitin chains that are utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes displaying the same fold but lack the active site cysteine of the E2. The UBE2N/UBE2V2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3 ligase.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 17,138.1 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 20 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme

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