UB218: UBE2N

SKU UB-0218 Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes

LifeSensors UB218 (UBE2N) is an E2 ubiquitin-conjugating enzyme that assembles K63-linked polyubiquitin chains, key in non-degradative ubiquitin signaling pathways like DNA repair and immune response. Ideal for research in ubiquitination and cellular signaling

Price range: $245.00 through $439.00

UBE2N (homologous to yeast Ubc13) is an E2 conjugating enzyme that forms a heterodimeric complex with the ubiquitin E2 variant (UEV) UBE2V2 (also known as MMS2 or UEV-2). This complex is responsible for the ubiquitylation of target substrates along with E1 and E3 enzymes. The UBE2N/UBE2V2 complex assembles K63-linked polyubiquitin chains that are utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes displaying the same fold but lack the active site cysteine of the E2. The UBE2N/UBE2V2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3 ligase.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	17,138.1 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation	20 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Applications

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

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UB218: UBE2N

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