UB204H: UBE2K (UbcH1), His6




UbcH1 (also known as E2-25K) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UbcH1 is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain.


Species Human
Source E. coli
Tag His6
Molecular Weight 23,400.7 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Storage -80°C, avoid freeze/thaw cycles

Additional information


0.8 nmol, 3 nmol


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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes


  • Untagged (native) E2 conjugation enzyme