Specifications
UbcH1 (also known as E2-25K) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UbcH1 is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain.
Info
Species | Human |
Source | E. coli |
Tag | His6 |
Molecular Weight | 23,400.7 Da |
Quantity | 0.8 nmol, 3.0 nmol |
Concentration | 40 µM |
Formulation | |
Storage | -80°C, avoid freeze/thaw cycles |
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