UB212: UBE2E2

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Specifications

UBE2E2 is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. It is a protein that in humans is encoded by the UBE2E2gene. Amino acid sequence within the UBC domain of UBE2E2 shares over 90% identity with human UbcH6, mouse UbcM2, and Drosophila UbcD2, whereas the N-terminal region shows little amino acid sequence similarity with known proteins. UBE2E2 forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 22,255.3 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme

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