UB200 E2 Selection Panel

UB200: E2 Selection Panel

$165.00

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Specifications

When initiating studies with a particular E3 ligase, UBE2D3 (UbcH5c) is often selected due to the reported promiscuity of this E2. However, it should be noted that several E3 ligases (including Parkin) have been reported not to work with UBE2D3. UBE2L3 (UbcH7) has been shown to function with several E3 ligases, including E6-AP and Parkin. UBE2N/UBE2V2 functions as a heterodimeric E2 enzyme for the production of K63-linked ubiquitin chains, and it is a good choice if you know that the E3 of interest builds this linkage type. This information can be a starting point in matching E2/E3 enzyme pairs for your ligase assays, but comprehensive profiling is still sorely needed.

The E2 Selection Panel is designed to provide the most flexible and cost-effective solution to this daunting task. In a single package, four* E2 conjugation enzymes from our catalog can be selected**. These enzymes can be used as stand-alone products or in conjugation with both the E3LITE Customizable Ubiquitin Ligase Kit or the E2 Profiling Kit.

* To order more than four E2s, please inquire about the price.
** Several E2s are available with His6SUMO tag or without tag.
*** Appropriate datasheets for selected E2 enzymes will be included with purchase.

This product is patented. Please click here to learn more.

Info

Panel Contents Each E2 Selection Panel comes with (4) E2 Conjugating Enzymes (0.8 nmol each)
Stability All components are stable for 6 months under recommended storage conditions
Storage -80°C, avoid freeze/thaw cycles

Additional information

E2 Conjugating Enzyme 1

UB201-0.8 NMOLES UBE2D3 (UbcH5c), UB202-0.8 NMOLES UBE2L3 (UbcH7, E2-F1), UB204-0.8 NMOLES UBE2K (UbcH1), UB204H-0.8 NMOLES UBE2K (UbcH1), His6, UB205-0.8 NMOLES Ubc13, UB206-0.8 NMOLES Mms2 (yeast), UB207H-0.8 NMOLES UBE2D2 (UbcH5b, E2-17K2), UB208H-0.8 NMOLES UBE2R1 (CDC34), His6, UB209-0.8 NMOLES UBE2R2 (Ubc3B), UB210T-0.8 NMOLES UBE2D1 -His6 SUMO tagged, UB212- 0.8 NMOLES UBE2E2, UB213-0.8 NMOLES UBE2E3, UB215H-0.8 NMOLES UBE2H, His6, UB215T-0.8 NMOLES UBE2H, His6SUMO, UB216-0.8 NMOLES UBE2F, UB217H-0.8 NMOLES UBE2M (Ubc12), His6, UB218-0.8 NMOLES UBE2N, UB219-0.8 NMOLES UBE2T, UB219T-0.8 NMOLES UBE2T, His6SUMO, UB220-0.8 NMOLES UBE2V2 (MMS2, UEV-2), UB221-0.8 NMOLES UBE2S, UB222-0.8 NMOLES UBE2C (Ubc10), UB223H-0.8 NMOLES UBE2W,His6, UB225-0.8 NMOLES UBE2Q2, UB226H-0.8 NMOLES UBE2A, His6, UB227-0.8 NMOLES UBE2G2, UB227T-0.8 NMOLES UBE2G2, His6SUMO, UB228H-0.8 NMOLES UBE2I, His6, UB229-0.8 NMOLES UBE2L6, UB230-0.8 NMOLES UBE2Z

E2 Conjugating Enzyme 2

UB201-0.8 NMOLES UBE2D3 (UbcH5c), UB202-0.8 NMOLES UBE2L3 (UbcH7, E2-F1), UB204-0.8 NMOLES UBE2K (UbcH1), UB204H-0.8 NMOLES UBE2K (UbcH1), His6, UB205-0.8 NMOLES Ubc13, UB206-0.8 NMOLES Mms2 (yeast), UB207H-0.8 NMOLES UBE2D2 (UbcH5b, E2-17K2), UB208H-0.8 NMOLES UBE2R1 (CDC34), His6, UB209-0.8 NMOLES UBE2R2 (Ubc3B), UB210T-0.8 NMOLES UBE2D1 -His6 SUMO tagged, UB212- 0.8 NMOLES UBE2E2, UB213-0.8 NMOLES UBE2E3, UB215H-0.8 NMOLES UBE2H, His6, UB215T-0.8 NMOLES UBE2H, His6SUMO, UB216-0.8 NMOLES UBE2F, UB217H-0.8 NMOLES UBE2M (Ubc12), His6, UB218-0.8 NMOLES UBE2N, UB219-0.8 NMOLES UBE2T, UB219T-0.8 NMOLES UBE2T, His6SUMO, UB220-0.8 NMOLES UBE2V2 (MMS2, UEV-2), UB221-0.8 NMOLES UBE2S, UB222-0.8 NMOLES UBE2C (Ubc10), UB223H-0.8 NMOLES UBE2W,His6, UB225-0.8 NMOLES UBE2Q2, UB226H-0.8 NMOLES UBE2A, His6, UB227-0.8 NMOLES UBE2G2, UB227T-0.8 NMOLES UBE2G2, His6SUMO, UB228H-0.8 NMOLES UBE2I, His6, UB229-0.8 NMOLES UBE2L6, UB230-0.8 NMOLES UBE2Z

E2 Conjugating Enzyme 3

UB201-0.8 NMOLES UBE2D3 (UbcH5c), UB202-0.8 NMOLES UBE2L3 (UbcH7, E2-F1), UB204-0.8 NMOLES UBE2K (UbcH1), UB204H-0.8 NMOLES UBE2K (UbcH1), His6, UB205-0.8 NMOLES Ubc13, UB206-0.8 NMOLES Mms2 (yeast), UB207H-0.8 NMOLES UBE2D2 (UbcH5b, E2-17K2), UB208H-0.8 NMOLES UBE2R1 (CDC34), His6, UB209-0.8 NMOLES UBE2R2 (Ubc3B), UB210T-0.8 NMOLES UBE2D1 -His6 SUMO tagged, UB212- 0.8 NMOLES UBE2E2, UB213-0.8 NMOLES UBE2E3, UB215H-0.8 NMOLES UBE2H, His6, UB215T-0.8 NMOLES UBE2H, His6SUMO, UB216-0.8 NMOLES UBE2F, UB217H-0.8 NMOLES UBE2M (Ubc12), His6, UB218-0.8 NMOLES UBE2N, UB219-0.8 NMOLES UBE2T, UB219T-0.8 NMOLES UBE2T, His6SUMO, UB220-0.8 NMOLES UBE2V2 (MMS2, UEV-2), UB221-0.8 NMOLES UBE2S, UB222-0.8 NMOLES UBE2C (Ubc10), UB223H-0.8 NMOLES UBE2W,His6, UB225-0.8 NMOLES UBE2Q2, UB226H-0.8 NMOLES UBE2A, His6, UB227-0.8 NMOLES UBE2G2, UB227T-0.8 NMOLES UBE2G2, His6SUMO, UB228H-0.8 NMOLES UBE2I, His6, UB229-0.8 NMOLES UBE2L6, UB230-0.8 NMOLES UBE2Z

E2 Conjugating Enzyme 4

UB201-0.8 NMOLES UBE2D3 (UbcH5c), UB202-0.8 NMOLES UBE2L3 (UbcH7, E2-F1), UB204-0.8 NMOLES UBE2K (UbcH1), UB204H-0.8 NMOLES UBE2K (UbcH1), His6, UB205-0.8 NMOLES Ubc13, UB206-0.8 NMOLES Mms2 (yeast), UB207H-0.8 NMOLES UBE2D2 (UbcH5b, E2-17K2), UB208H-0.8 NMOLES UBE2R1 (CDC34), His6, UB209-0.8 NMOLES UBE2R2 (Ubc3B), UB210T-0.8 NMOLES UBE2D1 -His6 SUMO tagged, UB212- 0.8 NMOLES UBE2E2, UB213-0.8 NMOLES UBE2E3, UB215H-0.8 NMOLES UBE2H, His6, UB215T-0.8 NMOLES UBE2H, His6SUMO, UB216-0.8 NMOLES UBE2F, UB217H-0.8 NMOLES UBE2M (Ubc12), His6, UB218-0.8 NMOLES UBE2N, UB219-0.8 NMOLES UBE2T, UB219T-0.8 NMOLES UBE2T, His6SUMO, UB220-0.8 NMOLES UBE2V2 (MMS2, UEV-2), UB221-0.8 NMOLES UBE2S, UB222-0.8 NMOLES UBE2C (Ubc10), UB223H-0.8 NMOLES UBE2W,His6, UB225-0.8 NMOLES UBE2Q2, UB226H-0.8 NMOLES UBE2A, His6, UB227-0.8 NMOLES UBE2G2, UB227T-0.8 NMOLES UBE2G2, His6SUMO, UB228H-0.8 NMOLES UBE2I, His6, UB229-0.8 NMOLES UBE2L6, UB230-0.8 NMOLES UBE2Z

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Applications

  • Identify cognate E2 enzymes for E3 ligases
  • Demonstration of novel E2 or E3 activity with cognate enzyme
  • High-throughput screening (HTS) of agonists/antagonists of either E2 or E3 activity
  • Determination of Michaelis constant (Km) for E2:E3 interactions (See E2:E3 Kinetic Application Note)
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