UB223H: UBE2W, His6

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Specifications

UBE2W is a ubiquitin conjugating enzyme that mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase. UBE2W was originally identified as the human homolog of the Arabidopsis E2 enzyme Ubc-16. Researchers demonstrated that UBE2W contains a NLS sequence and is localized to the nucleus of HEK293 cells. UBE2W binds to the E3 ligase BRCA1 and mediates the monoubiquitylation of BRCA12. In vitro UBE2W catalyzes monoubiquitination and ‘Lys-11’-linked polyubiquitination.

Info

Species Human
Source E. coli
Tag His6
Molecular Weight 18,456 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40µM
Formulation TBS, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

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