UB213: UBE2E3

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Specifications

UBE2E3 is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. The deduced 207-amino acid protein shares over 94% amino acid identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. Of these, however, only UbcM2 (thought to be the mouse homolog) exhibits homology in the N-terminal extension. UBE2E3 forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 22,913 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 20 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme

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