Ubiquitin Enzyme Assay

UC104: E2 SELECT Profiling & Selection Kit

$495.00

Choose one of each of the following to create your kit

Specifications

The E2 SELECT™ Profiling & Selection Kit allows for assessing the E2-E3 functional relationship. Therefore, it allows for identifying the optimal E2-conjugating enzyme for an E3 ligase of interest. The assay is based on the use of a plate that is pre-coated with a proprietary polyubiquitin-capture reagent. The E1 enzyme and ubiquitin are provided in the plate along with twenty-four E2 enzymes. Addition of ATP and of the E3 ligase of interest initiates the polyubiquitination process. The resulting polyubiquitin chains are captured in the wells and are detected in a chemiluminescent “sandwich” ELISA-like assay.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Kit Components:

  • 1 x pre-coated 96-well plate containing a mix of E1, E2, and Ubiquitin. 24 E2s are provided in triplicate.
  • E1-E2-E3 Control
  • Linear Polyubiquitin Detection Control
  • Detection Reagent 1

 

Info

Storage Store at -80°C
Stability Each plate is individually prepared. Use the kit within one month.

Additional information

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Applications

  • Testing functionality of over 24 E2’s with an E3 ligase of interest
  • Ready-to-assay.  Contains 24 human E2’s pre-plated in triplicate with E1 and Ubiquitin.
  • Detection system provides robust readout for E3 ligase activity.
  • E2 SELECT™ Kit utilizes non-radioactive reporter substrates.
  • Uses native, rather than tagged, ubiquitin

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