Specifications
The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convenient endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin.
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Components:
- pY-secSUMOstar vector (Cat.# 2161) — 20 µg
- SUMOstar Protease (Cat.# 4110) — 500 Units
- SUMOstar Protease control protein (Cat.# 5010) — 100 µg
- Anti-SUMOstar antibody (Cat.# AB7002) — 50 µg
Info
|
Fusion tag |
His6-SUMOstar |
|
Quantity |
n/a |
|
Promoter |
AOX1 |
|
Selection marker |
Ampicillin (E. coli), Zeocin (P. pastoris) |
|
Host |
E. coli, P. pastoris |
|
Storage |
Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles. |
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