1100A- pE-SUMOstar Amp Expression Kit

PY2101: pY-SUMOstar Saccharomyces Intracellular Vector

$430.00

In stock

SKU: PY-2101-0020 Category: Tags: ,

Specifications

The pY-SUMOstar vector allows for the intracellular expression of SUMOstar fusion proteins in Saccharomyces cerevisiae. SUMOstar fusion constructs are not processed in vivo, allowing for the same enhancement of protein expression, solubility, and stability afforded by SUMOpro.Protein expression is driven by the S. cerevisiae copper metallothionein (CUP1) promoter, which is both tightly regulated and highly inducible.  The plasmid is maintained by the 2-micron element and selected for by the restoration of L-tryptophan biosynthesis in Trp auxotrophs.

By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

  • pY-SUMOstar vector (Cat.# 2101) — 20 µg
  • SUMOstar Protease 1 (Cat.# 4110) — 500 Units
  • SUMOstar Protease control protein (Cat. #5010) — 100 µg
  • Anti-SUMO/SUMOstar antibody (Cat.# AB7002) — 50 µg

Info

Fusion tag 

His6-SUMOstar 

Quantity 

n/a 

Promoter 

CUP1

Selection marker 

Ampicillin (E. coli), Trp1 (yeast) 

Host 

E. coli, yeast 

Storage 

Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Additional information

SKU

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Applications

  • Enhanced intracellular expression of recombinant proteins as SUMOstar fusion proteins
  • Analysis of SUMOstar fusion protein expression
  • Removal of SUMOstar fusion tag for purification of native protein of interest

Benefits

  • SUMOstar enhances expression compared to traditional gene fusion systems.
  • Cleavage generates native protein, with desired N-terminus (except proline).

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