Other protein expression and purification systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest. LifeSensors’ highly active and specific SUMOstar Protease 1 recognizes the tertiary structure of the SUMOstar tag, generating the desired N-terminus and never cleaving within your protein of interest. In addition the SUMOstar system makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMOstar fusion tag and SUMOstar Protease 1, the SUMOstar system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.
This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.
|Promoter||T7 RNA polymerase|
|Selection marker||Ampicillin, Trp1|
|Host||E. coli, yeast|
|Storage||Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.|