SP4010: SUMO Protease 1

$295.00$4,265.00

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Specifications

For removal of SUMO (smt3) tags from fusion proteins expressed using SUMOpro systems (Cat#s: 1000A, 1000K, 1001A, 1001K). LifeSensors’ industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO. As a result, SUMO Protease 1 will not cleave within the fused protein of interest. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

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Info

Species Human
Source E. coli
Tag His
Molecular Weight 26 kDa
Quantity 250, 500, 1000, 5000 units
Concentration Variable
Formulation 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Units

250 units, 500 units, 1000 units, 5000 units

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Applications

  • Digestion and removal of SUMOpro tags from recombinant fusion proteins made with our SUMOpro Expression systems (Cat#s: 1000A1000K1001A1001K)

Benefits

  • SUMO Protease 1 cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5 – 9.5], and ionic strength. SUMO Protease 1 contains a polyhistidine tag at the N-terminus, making it easy to remove from the cleavage reaction by affinity chromatography.

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