For removal of SUMO (smt3) tags from fusion proteins expressed using SUMOpro systems (Cat#s: 1000A, 1000K, 1001A, 1001K). LifeSensors’ industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO. As a result, SUMO Protease 1 will not cleave within the fused protein of interest. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.
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|Molecular Weight||26 kDa|
|Quantity||250, 500, 1000, 5000 units|
|Formulation||50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol|
|Storage||-80°C, avoid freeze/thaw cycles|