1100A- pE-SUMOstar Amp Expression Kit

PY2160: pY Pichia Secretory SUMOstar Expression Kit

$1,570.00

In stock

SKU: PY-2160-1000 Category: Tags: ,

Specifications

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convenient endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin.

This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

  • pY-secSUMOstar vector (Cat.# 2161) — 20 µg
  • SUMOstar Protease (Cat.# 4110) — 500 Units
  • SUMOstar Protease control protein (Cat.# 5010) — 100 µg
  • Anti-SUMOstar antibody (Cat.# AB7002) — 50 µg

Info

Fusion tag 

His6-SUMOstar 

Quantity 

n/a 

Promoter 

AOX1 

Selection marker 

Ampicillin (E. coli), Zeocin (P. pastoris)

Host 

E. coli, P. pastoris

Storage 

Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Additional information

SKU

Reviews

There are no reviews yet.

Be the first to review “PY2160: pY Pichia Secretory SUMOstar Expression Kit”

Applications

  • Enhancing functional protein expression in P. Pastoris.

Benefits

  • SUMOstar fusion often enhances expression.
  • SUMOstar fusion often enhances protein solubility.
  • No known case of SUMOstar Protease cleaving within the fused protein-of-interest.
  • Cleavage yields native protein with a desired N-terminus.

Documents