1100A- pE-SUMOstar Amp Expression Kit

PY2161: pY-SUMOstar Pichia Secretory Vector

$430.00

In stock

SKU: PY-2161-0020 Category: Tags: ,

Specifications

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convient endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin.

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Info


Fusion tag 

His6-SUMOstar 

Quantity 

20 µg 

Promoter 

AOX1 

Selection marker 

Ampicillin (E. coli), Zeocin (P. pastoris)

Host 

E. coli, P. pastoris   

Storage 

-20°C 

 

Additional information

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Applications

  • Enhancing functional protein expression in P. Pastoris.

Benefits

  • SUMOstar fusion often enhances expression.
  • SUMOstar fusion often enhances protein solubility.
  • No known case of SUMO Protease 1 cleaving within the fused protein-of-interest.
  • Cleavage yields native protein with a desired N-terminus.

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