1100A- pE-SUMOstar Amp Expression Kit

PY2100: pY-SUMOstar Saccharomyces Intracellular Expression Kit

$1,570.00

In stock

SKU: PY-2100-1000 Category: Tags: ,

Specifications

In eukaryotes, endogenous SUMO proteases rapidly cleave SUMO fusions in vivo, precluding tag-enhanced expression and purification capacities. To circumvent this problem, LifeSensors has engineered the SUMOstar fusion system. SUMOstar offers the same solubility and expression enhancing benefits as the original wild-type SUMO tag; however, the tag is not cleaved by endogenous eukaryotic SUMO proteases. In addition, LifeSensors has created a cognate SUMOstar protease for specific  recognition and cleavage of the SUMOstar tag in vitro, liberating the protein-of-interest with a desired N-terminal amino acid. The pY-Saccharomyces Intracellular SUMOstar Kit allows for expression of SUMOstar fusion proteins intracellularly.

This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

  • pY-SUMOstar vector (Cat.# 2101) — 20 µg
  • SUMOstar Protease 1 (Cat.# 4110) — 500 Units
  • SUMOstar Protease control protein (Cat. #5010) — 100 µg
  • Anti-SUMO/SUMOstar antibody (Cat.# AB7002) — 50 µg

 

Info

Fusion tag 

His6-SUMOstar 

Quantity 

n/a 

Promoter 

CUP1

Selection marker 

Ampicillin, Trp1  

Host 

E. coli, yeast 

Storage 

Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Additional information

SKU

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Applications

  • Enhanced intracellular expression of recombinant proteins as SUMOstar fusion proteins.
  • Analysis of SUMOstar fusion protein expression.
  • Removal of SUMOstar fusion tag for purification of native protein of interest.

Benefits

  • SUMOstar enhances expression compared to traditional gene fusion systems.
  • Cleavage generates native protein, with desired N-terminus (except proline).

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