UM101-200µg Anti-Ub Tube1, GST

UM201: TUBE 1 (His6)

$375.00$1,105.00

Clear

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitinated state. The ubiquitination state of certain cellular proteins can be directly related to various diseases including neurodegeneration, inflammation and cancer. However, ubiquitinated proteins are intrinsically unstable, which precludes the characterization of the ubiquitinylation state in many cases. TUBEs will fill this void in ubiquitin research, allowing the identification of ubiquitin-modified proteins that cannot be detected with current technologies. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cells, tissues, and organs.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Tag

His6

Molecular Weight

37.7 kDa

Physical State

Liquid

Quantity

200 µg, 1 mg

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

Additional information

Weight

200 µg, 1 mg

Reviews

There are no reviews yet.

Be the first to review “UM201: TUBE 1 (His6)”

Applications

  • Pull down of poly-ubiquitylated proteins from cell and tissue lysates
  • Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome

Benefits

  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
  • Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
  • Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins

Documents