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Questions about a product, technology or experimental design? Give us a call 610-644-8845.

UB228H: UBE2I, His6

LifeSensors UB228H is a His6-tagged UBE2I (Ubc9) SUMO-conjugating enzyme that mediates transfer of SUMO1-4 from E1, essential for studying sumoylation in protein regulation.

Price range: $189.00 through $375.00

This E2 is a SUMO-conjugating enzyme, also known as Ubc9. UBE2I can mediate the transfer of SUMO1, SUMO2, SUMO3, and SUMO4 from SUMO-activating enzyme E1 (UBLE1A-UBLE1B complex) to a substrate protein. This E2 enzyme has been shown to associate with the SUMO E3 ligases, RANBP2 or CBX4. This E2 conjugating enzyme is necessary for sumoylation and it is essential for nuclear architecture and chromosome segregation.

Info

Species Human
Source E. coli
Tag His6
Molecular Weight 18565.3 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles
 
  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

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