UB219T: UBE2T, His6-SUMO

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Specifications

UBE2T can mediate the transfer of ubiquitin from ubiquitin-activating enzyme E1 to a substrate protein or E3 ligase. This E2 enzyme has been shown to be essential for the Fanconi anemia pathway. UBE2T conjugating activity is involved with the monoubiquitylation of FANCD2, co-functioning with the E3 ligase FANCL.

Info

Species Human
Source E. coli
Tag His6-SUMO
Molecular Weight 34,540.3 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 25 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes