UB202: UBE2L3 (UbcH7, E2-F1)

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Specifications

UBE2L3 (also known as UbcH7 or E2-F1) is an E2 conjugating enzyme involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UbcH7 has been shown to work with HECT domain E3s (E6-AP), in addition to RING containing E3s. UbcH7 is homologous to the UbcH5 family.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 17,861.6 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme