Specifications
UBE1, the ubiquitin activating enzyme, is the first enzyme in the pathway for conjugation of ubiquitin to target substrates through a ubiquitin-protein isopeptide bond. The C-terminal glycine of a ubiquitin (G76) is initially adenylated in an ATP dependent manner, followed by activation through transfer to the active site cysteine of UBE1, forming a ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) ubiquitin is then transferred to a specific lysine on a target substrate.
Info
| Species | Human |
| Source | E. coli |
| Tag | Untagged |
| Molecular Weight | 118 kDa |
| Quantity | 50 µg |
| Concentration | Variable |
| Formulation | 50mM Tris pH 8.0, 100mM NaCl, 10% glycerol |
| Storage | -80°C, avoid freeze/thaw cycles |
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