SI210: Single Lysine Ubiquitin Explorer Panel

LifeSensors SI210 is a Single Lysine Ubiquitin Explorer Panel providing ubiquitin K to R mutants for advanced protein ubiquitination research and analysis.

$788.00

SKU SI-0210-0000 Categories Research Kits, Ubiquitin K to R mutants

In stock

Product Advantages

Characterization of proteins responsible for ubiquitin conjugation or removal
Ligand binding studies with ubiquitin related proteins
Characterization of linkage type for target protein
profiling of E2/E3 interactions
Characterization of E3 polyubiquitylation activity
Tag-free recombinant ubiquitin

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to the primary amine groups of a lysine residue on target proteins. This covalent attachment is referred to as monoubiquitylation. Additional ubiquitin moieties can subsequently be conjugated to this initial ubiquitin, utilizing any one (of seven) lysine residue on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. LifeSensors has produced a complete panel of tag-free recombinant human ubiquitins that are engineered to have all available lysines mutated to arginines except at position mentioned. For example, ubiquitin-K6 has all available lysines mutated to arginines except at position 6, such that this molecule can only form polyubiquitin chains of K6-linkage type. The panel consists Ub mutants- K6, K11, K27, K29, K33, K48, and K63. The panel also contains WT Ub and ubiquitin-K0 (all lysines mutated to arginines).

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	Variable
Quantity	1 mg each
Concentration	Variable
Formulation	PBS
Storage	-80°C, avoid freeze/thaw cycles