SI209: Ubiquitin-K0


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SKU: SI-0209-1000 Category:


Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one (of seven) lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines. This molecule will not participate in polyubiquitin formation, and can therefore be used in control experiments for the study of ubiquitylation.


Species Human
Source E. coli
Tag None
Molecular Weight 8,760 Da
Quantity 1 mg
Concentration Variable
Formulation 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

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  • Characterization of proteins responsible for ubiquitin conjugation or removal.
  • Ligand binding studies with ubiquitin related proteins.
  • Characterization of linkage type for target protein.
  • Profiling of E2/E3 interactions.
  • Characterization of E3 polyubiquitylation activity.
  • Can be used as a negative control for ubiquitylation studies.
  • Native (tag free) recombinant wild-type ubiquitin.