SI0104B: Biotinylated M1 (linear) Tetra-Ubiquitin

Please Contact Us for Pricing!

Contact Us


M1-linked ubiquitination is traditionally associated with regulation of NFĸB and IFN signaling. M1 Tetra-Ubiquitin is a tetrameric chain of wild-type ubiquitin. It is expressed as a linear chain wherein monomers are linked through N-terminal methionines and C-terminal glycines. Increasing evidence suggests that polyUb chains joined through linear peptide bonds between the C-terminus of a ubiquitin and the N-terminus of another play important functional roles. The enzyme machinery responsible for the generation of linear polyUb chains has been termed LUBAC, consisting of HOIL-1L and HOIP. Chains of these type have been determined to have an open conformation, similar to polyUb K63, but with very distinct functional properties. Linear polyUb chains are cleaved by the deubiquitylases CYLD, USP5 (IsoT), USP2 and have been shown to bind to many UBDs including NEMO and Trabin-n (3xnzf).

This is recombinant tetraubiquitin expressed as a linear chain. Amide linkages join the N- and C-terminus of each ubiquitin molecule to each other. This molecule is HIS-tagged at the N-terminus of the most distal ubiquitin. With the M1 Linear Tetra Ubiquitin made, we then biotinylate it once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or vidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off of intended application (EX: DUB activity/specificity).


Species Human
Source E. coli
Tag Biotin
Molecular Weight 35,146.5 Da
Quantity Variable
Concentration Variable
Formulation 20 mM Tris pH 7.5, 150 mM NaCl
Storage -80°C, avoid freeze/thaw cycles

Additional information



There are no reviews yet.

Be the first to review “SI0104B: Biotinylated M1 (linear) Tetra-Ubiquitin”
  • Biotinylated chains are meant to label the protein so they can be selectively separated via a multitude of methods such as Western Blotting, pull downs (see website), ELISAs, etc.
  • Can use streptavidin or avidin to visualize this protein specifically.
  • Investigation of ubiquitin chain specificity and selectivity
  • Studies on the role of ubiquitin chains in protein degradation pathways (e.g., proteasomal and autophagic degradation)
  • Analysis of ubiquitin-mediated signaling pathways and cellular responses
  • Structural studies to elucidate the architecture and dynamics of ubiquitin chains
  • Screening assays to identify modulators of ubiquitin chain assembly and disassembly processes