13C, 15N, and 2H heavy-labeled ubiquitin provides a stable standard for ubiquitin structural study by NMR. The recombinant human ubiquitin is purified from E. coli expressing the human ubiquitin gene. The cells are grown in media where the sole nitrogen source is 15N-labeled ammonium sulfate, the sole carbon source is 13C-glucose, and respective amount of 2H is supplied. The recombinant human ubiquitin is purified without heating by a proprietary procedure that preserves the native structure of the protein. The purified protein is homogenous when analyzed by overloaded SDS gels. The pools of purified protein solution are extensively dialyzed against water and lyophilized.
|Quantity||5 mg, 10 mg|
|Storage||-20°C, avoid freeze/thaw cycles|