PY2161: pY-SUMOstar Pichia Secretory Vector

SKU PY-2161-0005 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

LifeSensors’ PY2161 is the pY-SUMOstar Pichia Secretory Vector enabling efficient expression and secretion of SUMOstar fusion proteins in Pichia pastoris yeast, boosting yield and solubility for research applications

$475.00

In stock

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His₆ tag preceeds SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of specific endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Fusion tag	His6-SUMOstar
Quantity	5µg
Promoter	AOX1
Selection marker	Ampicillin (E. coli), Zeocin (P. pastoris)
Host	E. coli, P. pastoris
Storage	-20°C

Enhancing functional protein expression in P. Pastoris

Benefits

SUMOstar fusion often enhances expression

SUMOstar fusion often enhances protein solubility

No known case of SUMO Protease 1 cleaving within the fused protein-of-interest

Cleavage yields native protein with a desired N-terminus

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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PY2161: pY-SUMOstar Pichia Secretory Vector

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