PY2161: pY-SUMOstar Pichia Secretory Vector

SKU PY-2161-0020 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

LifeSensors’ PY2161 is the pY-SUMOstar Pichia Secretory Vector enabling efficient expression and secretion of SUMOstar fusion proteins in Pichia pastoris yeast, boosting yield and solubility for research applications

$475.00

In stock

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His₆ tag preceeds SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of specific endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Fusion tag	His6-SUMOstar
Quantity	20µg
Promoter	AOX1
Selection marker	Ampicillin (E. coli), Zeocin (P. pastoris)
Host	E. coli, P. pastoris
Storage	-20°C

Enhancing functional protein expression in P. Pastoris

Benefits

SUMOstar fusion often enhances expression

SUMOstar fusion often enhances protein solubility

No known case of SUMO Protease 1 cleaving within the fused protein-of-interest

Cleavage yields native protein with a desired N-terminus

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

Phone (Office)

610.644.8845

Available Monday – Friday, 9:00 AM – 5:00 PM EST

Send Email

info@lifesensors.com

We typically respond within 24 hours

Visit Anytime

271 Great Valley Parkway Malvern, PA 19355

Send us a Message

Have a question about our solutions? We’d love to hear from you.

First Name *

Last Name *

Email Address *

Phone Number

Company/Organization

Subject*

Message *

I would like to receive updates about new products and research developments

* Required fields

Custom Solutions

Learning Center

PY2161: pY-SUMOstar Pichia Secretory Vector

Info

Benefits

Follow Us

Send us a Message

More from This Category

PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license.

Menu

Custom Solutions

Learning Center