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PA770: PROTAC® In Vitro Ubiquitination Assay Kit

Lifesensors PA770 is a PROTAC® In Vitro Ubiquitination Assay Kit for high-throughput screening of PROTAC activity by monitoring target protein ubiquitination with E3 ligases VHL, Cereblon, and HDM2.

$1,045.00

  • Testing PROTAC and molecular glue (MGs) activity.
  • Compare between multiple PROTAC variants and establish predictive DC50 from UbMax.
  • Choose all three ligases for comparing PROTACs / MGs activity to demonstrate selectivity.
  • Test PROTAC substrate specificity and Isoform selectivity.
Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict PROTAC efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor PROTAC mediated ubiquitination for target of choice. PROTAC In Vitro Ubiquitination Assay Kit Components
In Vitro ubiquitination Assay Plate 1 plate
Assay Buffer (10X) 1.2mL
E3 Mix (20X) 1 X 250µL
E1-E2-Ubiquitination Mix (20X) 1 X 250µL
Secondary Antibody (Anti-Mouse or Anti-Rabbit HRP conjugate) 60µL (100X)
Detection Reagent 1Detection Reagent 2 DR1- 1mLDR2- 1mL
Blocking Concentrate (5X) 5mL
PROTAC in DMSO 10µL
Target Protein positive control BRD3 (20X) 20µL
Anti-BRD3 antibody (100X) 5µL
Anti-mouse HRP Conjugate (100X) 5µL
This product has been cited 1 time. Need guidance or technical assistance with LifeSensors’ PROTAC Assay Plate? We’re happy to help! Contact our technical support team at customerservice@lifesensors.com. Learn more about PROTACs here.
  • Testing PROTAC and molecular glue (MGs) activity.
  • Compare between multiple PROTAC variants and establish predictive DC50 from UbMax.
  • Choose all three ligases for comparing PROTACs / MGs activity to demonstrate selectivity.
  • Test PROTAC substrate specificity and Isoform selectivity.

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PROTAC IS A REGISTERED TRADEMARK OF ARVINAS OPERATIONS, INC., AND IS USED UNDER LICENSE.