Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict PROTAC efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor PROTAC mediated ubiquitination for target of choice.
PROTAC In Vitro Ubiquitination Assay Kit Components
|In Vitro ubiquitination Assay Plate||1 plate|
|10X Assay Buffer||1.2mL|
|E3 Mix (20X)||1 X 250µL|
|E1-E2-Ubiquitination Mix (20X)||1 X 250µL|
|Secondary Antibody (Anti-Mouse or Anti-Rabbit HRP conjugate)||60µL (100X)|
|Detection Reagent 1 and 2||DR1- 1mL and DR2- 1mL|
|Blocking Concentrate (5X)||5mL|
|PROTAC in DMSO||10µL|
|Target Protein positive control BRD3 (20X)||20µL|
|Anti-BRD3 antibody (100X)||5µL|
|Anti-mouse HRP Conjugate (100X)||5µL|
Need guidance or technical assistance with LifeSensors’ PROTAC Assay Plate? We’re happy to help! Contact our technical support team at firstname.lastname@example.org.
Learn more about PROTACs here.
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