PA770: PROTAC® In Vitro Ubiquitination Assay Kit




Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict PROTAC efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor PROTAC mediated ubiquitination for target of choice.

PROTAC In Vitro Ubiquitination Assay Kit Components

In Vitro ubiquitination Assay Plate 1 plate
10X Assay Buffer 1.2mL
E3 Mix (20X) 1 X 250µL
E1-E2-Ubiquitination Mix (20X) 1 X 250µL
Secondary Antibody (Anti-Mouse or Anti-Rabbit HRP conjugate) 60µL (100X)
Detection Reagent 1

Detection Reagent 2

DR1- 1mL

DR2- 1mL

Blocking Concentrate (5X) 5mL
Target Protein positive control BRD3 (20X) 20µL
Anti-BRD3 antibody (100X) 5µL
Anti-mouse HRP Conjugate (100X) 5µL

Need guidance or technical assistance with LifeSensors’ PROTAC Assay Plate? We’re happy to help! Contact our technical support team at

Learn more about PROTACs here.

Additional information

PROTAC® In Vitro Ubiquitination Assay Kit

PA:770 Cereblon Kit, PA:770 VHL Kit, PA:770 HDM2 Kit

Secondary Antibody

Mouse, Rabbit


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  • Testing PROTAC and molecular glue (MGs) activity.
  • Compare between multiple PROTAC variants and establish predictive DC50 from UbMax.
  • Choose all three ligases for comparing PROTACs / MGs activity to demonstrate selectivity.
  • Test PROTAC substrate specificity and Isoform selectivity.