The UbiQuant™ S assay is a robust tool for assessing the poly-ubiquitination levels of a protein of interest in cell lysate. This platform is based on capturing poly-ubiquitinated proteins on the plate followed by detection of the protein of interest (either native or tagged) using an antibody for the purpose of quantifying the poly-ubiquitinated forms of the protein. Detecting changes in the ubiquitination state of specific proteins in response to external stimuli has been a long and difficult process involving immunoprecipitation followed by Western blot analysis. The UbiQuant™ S kit is a quick, robust, and quantitative alternative to IP/WB analysis.
Note: This kit has been optimized and validated for assessing the poly-ubiquitination levels of tagged proteins. Therefore, the end-user must specify the epitope tag in order to receive the appropriate antibody for detection ( myc, HA, FLAG®, V5, GST. Unfortunately, His6 cannot be used for this assay).