Otulin (Gumby) is a deubiquitinase exhibiting high specificity for linear ubiquitin chains (M1). Its specific interactions with the proximal ubiquitin leads to the activation of the catalytic triad. Otulin binds to HOIP (RNF31), a component of the linear chain synthesizing E3 ligase complex called LUBAC and regulates its activity thus affecting NFκB-regulated pathways and proinflammatory responses.
|Molecular Weight||40.6 kDa|
|Formulation||20 mM Hepes pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol|
|Storage||-80°C, avoid freeze/thaw cycles|