SP4020: SUMO Protease 2

$393.00 – $5,686.00

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SKU: SP-4020 Categories: , ,

Specifications

For removal of SUMOpro3 tags from fusion proteins expressed from the following SUMOpro3 expression systems: (Cat.#s: 1010A, 1016, 1010K, 1011, 2151). SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

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Info

Species Human
Source E. coli
Tag His6 
Molecular Weight 28 kDa
Quantity 250, 500, 1000, 5000 units
Concentration Variable
Formulation 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Units

250 UNITS, 500 UNITS, 1000 UNITS, 5000 UNITS

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Digestion and removal of SUMOpro tags from recombinant fusion proteins made with our SUMOpro Expression systems (Cat.#s: 1010A, 1016, 1010K, 1011, 2150, 2151)

Benefits

  • SUMO Protease 1 cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength. SUMO Protease 1 contains a polyhistidine tag at the N-terminus, making it easy to remove from the cleavage reaction by affinity chromatography.

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