SI3302: K33-Linked Di-Ubiquitin


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SKU: SI-3302-0025 Category: Tag:


K33-linked di-ubiquitin (Ub2) is a diubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 33 on the proximal ubiquitin. The product is generated using chemical ligation. It is a useful substrate for identifying and characterizing deubiquitinating enzymes that cleave the K33 linkage and for structural and binding studies of ubiquitin chain recognition by ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs).


Species Human
Source E. coli
Tag None
Molecular Weight 17112 Da
Quantity 25 µg
Concentration Variable
Formulation 20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA
Storage -80°C, avoid freeze/thaw cycles

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  • Investigation of DUB linkage specificity.