Specifications
Fluorescence polarization substrate for deubiquitylating enzymes. The substrate consists of a 5-carboxytetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide where the Ɛ-amino group of Lys is linked to the C-terminus of ubiquitin. The substrate allows continuous fluorescence polarization measurement of cleavage of the isopeptide bond between ubiquitin and the Ɛ-amino group of Lys. The rhodamine fluorophore minimizes interference from compounds and assay components that fluoresce at lower wavelengths.
Info
| Species | Human |
| Source | E. coli |
| Tag | None |
| Molecular Weight | 9162 Da |
| Quantity | 50 µg |
| Concentration | Variable |
| Formulation | Dry powder |
| Storage | -80°C, avoid freeze/thaw cycles |
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