SI0102-5µg (panel) Linear (Ub2) di-ubiquitin

SI270: Ubiquitin-epsilon-Lys-TAMRA-Gly-OH

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Specifications

Fluorescence polarization substrate for deubiquitylating enzymes. The substrate consists of a 5-carboxytetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide where the Ɛ-amino group of Lys is linked to the C-terminus of ubiquitin. The substrate allows continuous fluorescence polarization measurement of cleavage of the isopeptide bond between ubiquitin and the Ɛ-amino group of Lys. The rhodamine fluorophore minimizes interference from compounds and assay components that fluoresce at lower wavelengths.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 9162 Da
Quantity 50 µg
Concentration Variable
Formulation Dry powder
Storage -80°C, avoid freeze/thaw cycles

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  • Determining the activity and specificity of isopeptidases for Ub/Ubl-proteins
  • High Throughput Screening for modulators of DUB activity