Fluorescence polarization substrate for deubiquitylating enzymes. The substrate consists of a 5-carboxytetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide where the Ɛ-amino group of Lys is linked to the C-terminus of ubiquitin. The substrate allows continuous fluorescence polarization measurement of cleavage of the isopeptide bond between ubiquitin and the Ɛ-amino group of Lys. The rhodamine fluorophore minimizes interference from compounds and assay components that fluoresce at lower wavelengths.
|Molecular Weight||9162 Da|
|Storage||-80°C, avoid freeze/thaw cycles|