SI0102-5µg (panel) Linear (Ub2) di-ubiquitin

SI270T: Ubiquitin, TAMRA-labeled

$125.00

In stock

SKU: SI-270T-0100 Category: Tags: ,

Specifications

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains.  Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods.  Fluorescent-labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro.  Unlike others, LifeSensors’ TAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Info

Species Human
Source E. coli
Tag None
Molecular Weight  9133.5 Da
Quantity 100 µg
Concentration Variable
Formulation PBS
Storage -80°C, avoid freeze/thaw cycles

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  • In vitro detection of ubiquitin conjugation
  • Determination of the activity of ubiquitin conjugating enzymes