DU0601: K6-linked Diubiquitin (IQF)


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SKU: DU-0601-0050 Categories: ,


The diubiquitin substrates represent a new class of continuous assay substrates for the cleavage of a true isopeptide bond. They are based on the formation of an internally quenched fluorescent FRET pair (IQF) in which the fluorescence quencher is located on one ubiquitin moiety and the fluorescent dye is located on the second. The ubiquitins are joined by an isopeptide linkage between the C-terminal glycine of one ubiquitin and the e-amino group of lysine 6 residue in the second ubiquitin.

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Species Human
Source E. coli
Tag None
Molecular Weight 18,474 Da
Quantity 50 µg
Concentration Variable
Formulation 50 mM Sodium MES, pH 6.0.
Storage +4oC

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Real time measurement of isopeptide bond cleavage by a DUB.